Oregano is a plant utilized as a spice in numerous regions of the world due to its characteristic sensory attributes of flavor, color, and aroma. The use of oregano. This study evaluated growth patterns of Mexican oregano (Lippia berlandieri) under salt stress and tested the hypothesis that this species is salt tolerant. J Food Prot. Dec;74(12) doi: / Antifungal activity evaluation of Mexican oregano (Lippia berlandieri Schauer).
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Among them, carboxymethyl cellulose CMC and starch S are the most widespread and economic biomaterials. Moreover, these berlaneieri pointed out that EO effectiveness is influenced by the sensitivity of the microorganism. The main advantage of using EOs in ,ippia polymer matrix coating is a slow diffusion of the antimicrobial agent, leaving high concentrations of the active compound in contact with the beroandieri surface where contamination has occurred or is likely to occur, for a long period [ 20 ].
Inhibition curves of Listeria monocytogenes at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC a 0. One inconvenience of EO direct application on surfaces by dipping, powdering, or spraying is that antimicrobial compounds can be neutralized or diffuse rapidly from the surface into the product [ 30 ].
Data were fitted to the Fermi model [ 23 ] from which the biological parameters and were estimated by nonlinear regression using the KaleidaGraph 3. Antimicrobial assay was performed. Based on their traditional use, these extracts obtained from plants are first recommended, due to their broad-spectrum antimicrobial action and low side effects treatments [ 3 ].
Natural antimicrobial compounds effectiveness depends on the type, genus, species, and strain of the target microorganism and some food intrinsic and extrinsic factors such as pH, temperature, water content, atmospheric composition, and initial microbial load, with pH being one of the most important factors due to antimicrobial dissociation [ 3 ].
Thus, adequate pH values can change the membrane permeability and disintegrate the outer membrane of Gram-negative bacteria releasing lipopolysaccharides.
Several works in the literature focus on the evaluation of the antibacterial effect of EOs and propose them as an interesting option to ensure the safety and quality of minimally processed foods and extend their shelf life [ 1314 ]. This model describes a sigmoid decay and generates two biological parameters and between pH and MOEO concentration Table 1.
Therefore, CMC releases more active compounds. However, many reports mentioned that, for proper operation of active edible films, the optimal pH should be considered at which the antimicrobial agent may have a state of dissociation that allows better antimicrobial activity.
As observed, when MOEO concentration increased, a rapid microbial inactivation was observed, while at lower concentrations, the inactivation presented a remarkable nonlinear kinetics. Table of Contents Alerts.
MOEO chemical analysis showed that the two major compounds were thymol 2. Survival curves for both microorganisms are presented in Figures 1 and 2. Indexed in Science Citation Index Expanded. Moreover, the Fermi model can explain and predict microbial reduction in all tested pathogens and is suitable to describe inactivation in similar matrices and under different lipoia conditions in the future [ 26 ].
Inhibition curves of Staphylococcus aureus at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC a 0.
Lippia graveolens – Wikipedia
Moreover, it can be observed that, for both kinds of edible films, pH values of 5 and 6 are more effective than 7. Statistical analyses were performed with Minitab 17 software Minitab Inc. The difference between CMC and starch polymers is the release of the antimicrobial compounds mainly thymol and carvacrol present in MOEO.
Then, sorbitol Aldrich Chemical Co. Microbial Strains of L. San Claudio, Ciudad Universitaria, Col.
Journal of Food Quality
Microbial modeling has allowed estimating key intrinsic factors as pH and MOEO concentration with the synergistic effect against two important food-borne pathogens. Introduction Researchers around the world are investigating the use of essential oils to protect food from microbial growth.
The obtained spectra were compared with the respective mass spectra of pure compounds and with the mass profile of the same compounds available from the US National Institute of Standard Technology NIST library. Moreover, there are evidences that EOs incorporated to edible films can inhibit a large variety of food-borne pathogens [ 1315 — 19 ]. Edible Lppia Preparation Films were made according to the Bertuzzi et al.
Journal of Food Quality. Carvacrol 5-isopropylmethylphenol and thymol 2-isopropylmethylphenol are the major components of the essential oils of some species belonging to the Lamiaceae family including oregano, and these are phenolic compounds; isomers of monoterpenes exhibit significant antimicrobial activity in vitro [ 67 ]. In addition, its chemical, physical, and functional characteristics make them suitable to add essential oils EOs as antimicrobials [ 10 — 12 ].
This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, berkandieri, and reproduction in any medium, provided the original work is properly cited.
Our results demonstrate that edible films can serve as carriers releasing EO onto the surface controlling bacterial growth and reducing diffusion into the agar since the EO chemically forms part of the structure of the film and interacts with the polymer and the plasticizer [ 31 ].
Therefore, berpandieri investigations should be conducted for the application of CMC and starch as edible coatings with MOEO in food to confirm their antibacterial effect and mechanical and sensory properties.
Consortium of Intermountain Herbaria – Lippia berlandieri
Correspondence should be addressed to Raul Avila-Sosa ; xm. All tests were run in triplicate. The antimicrobial activity of essential oils and plant extracts is well known for a llippia time, and many research results have been published against food-borne pathogens [ 1 ] and lppia their low toxicity to mammals, fewer environmental effects, and low cost, which make them more attractive than synthetic antimicrobials [ 2 ]. Materials and Methods 2.
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