Br J Pharmacol. Oct;(4) doi: /j x. Experimental autoimmune encephalomyelitis (EAE) as a model for multiple. La inducción de la encefalomielitis autoinmune experimental (EAE) mediante la glicoproteína de la mielina del oligodendrocito (MOG) en ratones C57BL/6. Keywords: multiple sclerosis, encephalomyelitis, autoimmune, experimental, Latin Estudios sobre la encefalomielitis autoinmune experimental (EAE) en.

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Accepted in April, T H 17 cytokines in autoimmune neuro-inflammation. This procedure is not equally successful with every animal species, however —especially when using myelin oligodendrocyte glycoprotein MOG for this purpose— due to variations in genetic background and allele polymorphisms of class II histocompatibility complex molecules and T-cell receptors [9].


The typical susceptible rodent will debut with clinical symptoms around two weeks after immunization and present with a relapsing-remitting disease. Preparation of the antigen for inducing EAE FIA plus MT The contents of one ampoule of MT mg were transferred into a small mortar and the yellowish bacterial aggregates were ground into a whitish fine powder and then mixed under continuous agitation with According to the results, the diseased animals exhibited increased levels of IL mRNA, in agreement with the data published by other authors working on this model [34].

Leptin neutralization interferes with pathogenic T cell autoreactivity in autoimmune encephalomyelitis. Inresearchers identified a novel class of helper T cells called Th17 cells that seem to contribute to the pathogenesis of EAE and possibly MSalthough the precise mechanism remains unclear Korn et al. Usa los ratones no tratados como el grupo de control, para poder comparar enferma con animales sanos.

Experimental autoimmune encephalomyelitis (EAE) as a model for multiple sclerosis (MS).

However, its contribution to this endeavor is controversial: The characteristic ultrastructural changes of MS were also detected; namely, demyelination and axonal damage. The archetypical first clinical symptom is weakness of tail tonus that progresses to paralysis of the tail, followed by a progression up the body to affect the hind limbs and finally the forelimbs.


The experimental protocol was submitted for review to the institutional reviewing committee for the use and handling of laboratory animals of the Center for Genetic Eexperimental and Biotechnology CIGB, Cubaaccording to current international standards [12].

They were used after a laboratory adaptation period of one week. The difference between the observed body weight and that measured at day 0 of the study was determined daily, calculating group means to follow the behavior of this variable.

Experimental autoimmune encephalomyelitis (EAE) as a model for multiple sclerosis (MS).

The origin and application of experimental autoimmune encephalomyelitis. Nat Rev Drug Discov. If the problem continues, please let us know and we’ll try to help. Leptin is a hormonal peptide produced by adipocytes that participates in the regulation of energy balance and food dxperimental in the atoinmune [32].

An unexpected error occurred. Recently it has been found that CSF from MS patients can carry the disease to rodents, opening the door to an alternative model. This is a relatively early onset within the range of days post-induction described by other authors [26, 27].

A misleading model of Multiple Sclerosis”. Views Read Edit View history. Ultrastructural study by transmission electron microscopy At the end of the study period, three animals each were randomly selected from the negative control and placebo-treated EAE groups and sacrificed by cervical dislocation. EAE provides a means for investigating mechanisms of autoimmune-related CNS damage and demyelination.

Experimental Autoimmune Encephalomyelitis

Disease processes that can be studied EAE provides a powerful framework for investigating the inflammatory elements of MS. Un curso de la enfermedad representante de ratones EAE se muestra experimenyal la Figura 1. Textbook of Medical Physiology. The mixture was then transferred to a pair of 5 mL glass syringes connected to each other through a 16 G metal capillary, carefully avoiding the inclusion or formation of air bubbles inside the emulsion.


Se centrifuga a 2. Centrifugar durante 6 min a xg a temperatura ambiente RT. We suggest that body weight constitutes a significant clinical correlate only during the initial period of the disease, when the first clinical signs appear and this variable follows a downward trend. In the negative control group, body weight increased steadily throughout the study, at a rate above that of diseased animals.

Este curso se divide en tres fases: Retraction of any of the hind limbs to the abdominal region while hanging in this position constitutes one of the initial signs of the disease, and is associated with pathological processes in the spinal motor nerves, including axonal degeneration [22, 23]. In the early days of EAE, studying disease states caused by injecting brain homogenate in different species helped pinpoint how particular tissue-specific molecules produce autoimmune reactions Krishnamoorthy and Wekerle, When spinal cord samples were analyzed by transmission electron microscopy, the individuals from the negative control group exhibited compact, dense myelin sheaths and no signs of axonal damage Figure 4A.

Experimental autoimmune encephalomyelitis EAE.

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