BACTEC™ // I. PRINCIPLE. The detection of microorganisms in a patient’s blood has diagnostic and prognostic importance. Bactec Plus blood culture bottles were preincubated at 35°C or at room temperature before entry into the Bactec instrument to determine the influence of. The overall recovery of organisms and time to detection with the BACTEC and BACTEC systems were compared in a multicenter.
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Conversely, all Candida spp. The predominant cause of candidemia had been C. It is used for detecting the amount of bacterial contamination in platelet concentrates. Make a Call Today! With the increasing prevalence of candidemia among hospitalized patients, particularly secondary to NAC, further investigation is necessary to improve the recovery of all Bacgec spp.
However, when only standard aerobic and anaerobic media were used, the BacT performed better than the Bactec in overall growth detection, time to growth detection, and number of false-negatives. This unique medium contains a lysing agent to lyse both red and white blood cells, releasing any intracellular organisms.
Microbiology System — is well suited for use in large laboratories.
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Bactec 9240 Blood Culture System, BD Bactec 9240
Most of the Candida spp. Terminal subculture was performed on all bottles in which no growth was detected; a 0. Journal List J Clin Microbiol v.
A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The mean time to growth detection in the BacT system was Multifactor analysis of variance was followed by Student-Newman-Keuls tests.
BD BACTEC™ Blood Culture Media – BD
Growth was detected in all BacT and Bactec mycology bottles, all BacT aerobic bottles, and by terminal subculture of all bottles. Harrison2 and Duane R. Recall, though, that our isolates were all incubated for a total of 12 days.
Fifty clinical isolates of Candida were used in this study, including 10 isolates of each of the following species: The aerobic detection of C. However, the mean time to aerobic growth detection is strikingly slower for C. Both automated blood culture systems detected all episodes of simulated candidemia when specialized mycology media were used.
In the course of performing a separate study on simulated candidemia in the Bactec, we noted some difficulty in growth detection and time to growth detection among both C. A closer review of the data also reveals an important finding.
Interestingly, the only Candida sp. Individual blood culture bottles were removed from the automated blood culture systems when growth was detected and time to detection was recorded. Secular trend of hospital-acquired candidemia among intensive care unit patients in the United States during A prospective observational study of candidemia: Detection of Candida spp.
However, these studies address only a small number of candidemia episodes, primarily secondary to Candida albicans. Do you like this? The corresponding time to growth detection of C. Fresh, whole blood was drawn from healthy volunteers after obtaining written informed consent. This protocol was approved by the institutional review board at Brooke Army Medical Center.
Contact Sales Contact Support. We therefore performed this study to directly compare the ability of two automated blood culture systems in the detection of the five most common species implicated in candidemia, specifically evaluating growth detection, time to growth detection, and the utility of terminal subcultures with a simulated candidemia model.
For more information on the Bactec microbiological system and other refurbished microbiological systems we carry, please browse through our online store. Open in a separate window.
Murray1 Linda S. The BD Bactec system can accept test bottles, and comes complete with a rich set of features to provide maximum workflow and productivity.
It is therefore important to also look at the overlap of missed isolates to determine if there were any missed episodes of simulated candidemia.
De La Maza, and E. Each blood culture bottle was inoculated with fresh blood from healthy donors. Therefore, time to detection of Candida growth is an important consideration. Mean time to growth detection of C. However, few institutions routinely utilize a mycology bottle as a part of all blood culture sets, secondary to the requirement for extra blood and laboratory space.
The views expressed are those of the authors and do not reflect the official policy or position of the Department of the Army, Department of Defense, or the U. Apart from offering enhanced safety and simplified operation, it delivers excellent performance, media quality, reliability, and ease of use.
All bottles were subcultured bacec demonstrated heavy growth of pure yeast colonies and were therefore true positives.
Secular trends in the epidemiology of nosocomial fungal infections in the United States, The BacT system missed no episodes of candidemia. We feel these statistical differences do not likely bsctec clinically significant differences. Prior to inoculation with blood, the MB bottles were also inoculated with 1 ml of enrichment fluid as recommended by the manufacturer for use with this medium.