ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .
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Cancer Res 74 1: Ithimakin S et al.
Genetic or pharmacological inhibition of the leukotriene-generating enzyme arachidonate 5-lipoxygenase Alox5 abrogates neutrophil pro-metastatic activity and consequently reduces metastasis. Corti S et al. These reagents may also improve discrimination of the ALDHbr population, but results will vary by sample type. Noto A et al. Exp Hematol 35 9: Do erythrocytes red blood cells interfere with the assay?
Gerber JM et al. J Transl Med 8: Villablanca EJ et al. Cytometry B Clin Cytom 72 4: Nakamura S et al.
Obermair FJ et al. Abstract Pancreatic alcefluor adenocarcinoma PDAC is one of the deadliest cancers in western countries, with a median survival of 6 months and an extremely low percentage of long-term surviving patients.
Cancer Res 69 Abstract Mesenchymal stem or stromal cells MSCs have many potential therapeutic applications including therapies for cancers and tissue damages caused by cancers or radical cancer treatments.
Prostate Epithelial Cell Research. Sarcomas Sangiolo D et al. To circumvent aldeflour issues, we derived MSCs from transgene-free human induced pluripotent stem cells iPSCs efficiently with a modified protocol that eliminated the need of flow cytometric sorting.
Yamamoto M et al. Our integrated analyses illuminate a therapeutic strategy of combined targeting ikt the KRAS pathway and mitochondrial respiration to manage pancreatic cancer.
These issues hinder the potential applications of MSCs, especially those in cancer patients. All other trademarks and registered trademarks are the property of their respective holders.
Cord blood units may be collected into citrate phosphate dextrose anticoagulant. Adequate compensation will not be achieved with commercially available fluorescent beads. Muramoto GG et al. Herreros-Villanueva M et al. Human stem and progenitor cells typically have more ALDH activity than mature cells, and this quantitative difference allows stem cells to be resolved from the other cells.
What anticoagulants can be used to collect samples?
Lu J et al. Frequently Asked Questions The reagents in the kit were frozen when I received it. Liu P et al. Mesenchymal Cells Nagano M et al. Prostate Cells Yao M et zldefluor.
Safety Data Sheet 4. Kryczek I et al.
ALDEFLUOR™ Kit | STEMCELL Technologies
Research Area Workflow Stages for. Todaro M et al. Breast Cancer Res 15 5: Watch this video to see an example of how our scientists increased the fluorescence intensity of ALDH br cells in mammary tissue, and iit you can modify the protocol to fit your research needs.
Safety Data Sheet 2. For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. J Pathol 2: Identifies only viable cells with an intact cell membrane. Compatible with standard cell sorters or analyzers. Colon Cells Carpentino JE et al.